Description

Rolling wall vessels (RWV) utilizing both single cell lines and co-cultures with two or more cell types are contributing significantly to our understanding of tumor development, cell biology and the role of cell-cell interaction in physiological processes. These studies would be significantly enhanced if the various populations of cells could be individually identified and monitored over the course of experimentation. Optical and ultrasound technologies will be employed to monitor the development and structure of these constructs. Multiple studies could be further enhanced if it were possible to grow three dimensional constructs comprised of multiple human tissues in immunodeficient mice and image these with ultrasound and optical technologies. Constructs grown in mice may well develop far beyond what is currently possible in vitro. This could open the door for a multitude of previously impossible routes of investigation. We propose to introduce genes coding for firefly luciferase and GFP into various human cell types to allow each population to be monitored in real time over the course of experimentation. By using imaging technology to visualize and quantify different cell populations incorporated into the three dimensional constructs that are the result of RWV culture, our understanding of cell-cell interaction, anti-cancer drug efficacy and specificity and other aspects of cell biology will be greatly enhanced. By implanting these imagable three dimensional constructs into immunodeficient mice, it may be possible to continue growing these multicellular structures thereby affording researchers opportunities to study tumor and cell biology, cellular interaction and many other areas not currently possible.

General Information

• This data is described at

  http://catalog.data.gov/dataset/optical-imaging-of-three-dimensional-cell-constructs-grown-in-microgravity-and-in-immunode Do you think this data is incorrect? Let us know

• This data is published by

  National Aeronautics and Space Administration Do you think this data is incorrect? Let us know

Legal Information

• The rights statement is at

  http://catalog.data.gov/dataset/optical-imaging-of-three-dimensional-cell-constructs-grown-in-microgravity-and-in-immunode Do you think this data is incorrect? Let us know

• Outside the US, this data is available under

  Creative Commons CCZero Do you think this data is incorrect? Let us know

• There are

  yes, and the rights are all held by the same person or organisation Do you think this data is incorrect? Let us know

• The content is available under

  Creative Commons CCZero Do you think this data is incorrect? Let us know

• The rights statement includes data about

  its data licence Do you think this data is incorrect? Let us know

• This data contains

  no data about individuals Do you think this data is incorrect? Let us know

Practical Information

• The data appears in this collection

  http://catalog.data.gov/organization/nasa-gov Do you think this data is incorrect? Let us know

• The accuracy or relevance of this data will

  go out of date but it is timestamped Do you think this data is incorrect? Let us know

• The data is

  backed up offsite Do you think this data is incorrect? Let us know

Technical Information

• This data is published at

  http://techport.nasa.gov/xml-api/5443 Do you think this data is incorrect? Let us know

• This data is

  machine-readable Do you think this data is incorrect? Let us know

• The format of this data is

  a standard open format Do you think this data is incorrect? Let us know

Social Information

• The documentation includes machine-readable data for

  title Do you think this data is incorrect? Let us know

• The documentation includes machine-readable data for

  description Do you think this data is incorrect? Let us know

• The documentation includes machine-readable data for

  identifier Do you think this data is incorrect? Let us know

• The documentation includes machine-readable data for

  landing page Do you think this data is incorrect? Let us know

• The documentation includes machine-readable data for

  publisher Do you think this data is incorrect? Let us know

• The documentation includes machine-readable data for

  keyword(s) or tag(s) Do you think this data is incorrect? Let us know

• The documentation includes machine-readable data for

  distribution(s) Do you think this data is incorrect? Let us know

• The documentation includes machine-readable data for

  release date Do you think this data is incorrect? Let us know

• The documentation includes machine-readable data for

  modification date Do you think this data is incorrect? Let us know

• The documentation includes machine-readable data for

  temporal coverage Do you think this data is incorrect? Let us know

• The documentation includes machine-readable data for

  language Do you think this data is incorrect? Let us know

• The documentation about each distribution includes machine-readable data for

  release date Do you think this data is incorrect? Let us know

• The documentation about each distribution includes machine-readable data for

  a URL to access the data Do you think this data is incorrect? Let us know

• The documentation about each distribution includes machine-readable data for

  a URL to download the dataset Do you think this data is incorrect? Let us know

• The documentation about each distribution includes machine-readable data for

  type of download media Do you think this data is incorrect? Let us know

• Find out how to contact someone about this data at

  http://catalog.data.gov/dataset/optical-imaging-of-three-dimensional-cell-constructs-grown-in-microgravity-and-in-immunode Do you think this data is incorrect? Let us know

• Find out how to suggest improvements to publication at

  http://www.data.gov/issue/?media_url=http://catalog.data.gov/dataset/optical-imaging-of-three-dimensional-cell-constructs-grown-in-microgravity-and-in-immunode Do you think this data is incorrect? Let us know