CHLOROPHYLL A and BACTERIA - BACTERIAL DENSITY profile data collected in the South Pacific Ocean on the LAURENCE M. GOULD cruises LMG0104 and LMG0106 as part of the Southern Ocean GLOBEC project from 2001-04-29 to 2001-08-20 (NODC Accession 0112521)
Vydavatel National Oceanic and Atmospheric Administration, Department of Commerce
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NODC Accession 0112521 includes profile and biological data collected aboard the LAURENCE M. GOULD during cruises LMG0104 and LMG0106 in the South Pacific Ocean from 2001-04-29 to 2001-08-20. These data include CHLOROPHYLL A and BACTERIA - BACTERIAL DENSITY. The instruments used to collect these data include bottle. These data were collected by Christian H. Fritsen of University and Community as part of Southern Ocean GLOBEC. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NODC on 2013-07-23. The following is the text of the abstract provided by BCO-DMO: Bacteria Abundance, Biomass and Chlorophyll a Water Column Samples Contributor: Dr. Christian Fritsen University and Community College System of Nevada Desert Research Institute Div. of Earth and Ecosystem Sciences 2215 Raggio Parkway Reno, NV 89512 Office: 775/673-7487 BG 235 - Methods used for chlorophyll a (chla) analysis and bacteria biomass determination Core Sampling techniques: Sampling methods for recovery of chlorophyll a and bacteria from sea ice cores follows those described in: Garrison, D.L. and K.R. Buck(1986), Organism losses during ice melting: a serious bias in sea ice community studies. Polar Biol., 6:237-239. Recommendations for reporting were used as outlined by: Horner, R. et al.,(1992), Ecology of Sea Ice Biota. I: Habitat, Terminology and Methodology. Polar Biol. 12:417-427 Analytic Techniques: Chla (mg m-3): determined fluorometrically (Turner Designs 10AU Fluorometer) following extraction in 90% acetone (Parsons et al., 1984) ice core chla corrected to account for chla in filtered sea water (FSW) added to core sections during melting Bacteria cell abundance (cells m-3) and biomass (mg C m-3): LMG 0106 preserved (0.5% glutaraldehyde) samples stained with 4',6-diamidino-2-phenylindole (DAPI; 0.1 to 0.3% final concentration), filtered through 0.2 mm black, polycarbonate membrane filters, and mounted onto glass microscope slides on the ship (within 24 hours following collection) bacteria enumerated using epifluorescence microscopy and sized using digital images taken with Image Pro Plus bacteria biomass determined using cell abundance, cell biovolume (BV; mm3; as determined from mean length and width measurements), and an allometric conversion factor for bacterial carbon per volume specific for DAPI-stained bacteria (cellular carbon = 218 X BV0.86; Loferer-Kr��bacher et al., 1998). ice core samples corrected for FSW dilution NBP 0104 preserved (0.5% glutaraldehyde) samples stained with Sybr� Gold (0.01% final concentration), filtered through 0.2 mm Anodisc filters (Whatman�), and mounted onto glass microscope slides at home institution (~1-2 months following collection) bacteria enumerated using epifluorescence microscopy and sized using digital images taken with Image Pro Plus bacteria biomass determined using cell abundance, cell biovolume (BV; mm3), and an allometric conversion factor for bacterial carbon per volume specific for Acridine Orange-stained bacteria (cellular carbon = 89.9 X BV0.59; Simon and Azam, 1989). Note: an AO-specific carbon per volume conversion factor was used in calculating biomass in Sybr� Gold-stained samples because both AO and Sybr� Gold stain bacteria cells similarly relative to DAPI (unpublished data). ice core samples corrected for FSW dilution Loferer-Kr��bacher, M., Klima, J., and R. Psenner. 1998. Determination of bacterial cell dry mass by transmission electron microscopy and densitometric image analysis. Applied and Environmental Microbiology. 64:688-694. Parsons,T.R., Maita, Y., and C.M. Lalli. 1984. A manual of chemical and biological methods for seawater analysis. Pergamon Press. Elmsford, New York. Simon, M., and F. Azam. 1989. Protein content and protein synthesis rates of planktonic marine bacteria. Marine Ecology Progress Series. 51, 201-213. Data from LMG0106 (July-August, 2001) added in June 2002. Updated: April 21, 2006