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During April 2001 RoxAnn single-beam acoustic surveys were conducted at the North Unit of the Tortugas Ecological Reserve, Florida. Thirteen transects associated with fixed bottom sampling stations were surveyed to characterize the ecotone around the stations. In addition survey tracks were run along the outer edge of the Tortugas platform reef complex and into the interior of the platform. A towed video sled (minibat) was deployed simultaneously with the RoxAnn survey which provided a live video ground truth to the acoustic signals. Using this ground truth several bottom classes were identified including coral, seagrass, algae, unconsolidated sediments, and hardbottoms. The original RoxAnn categories are preserved in the Class field and benthic habitat codes from the Florida System for Classifying Estuarine and Marine Environments (SCHEME) system are preserved in the SCHEME field. The depth value is in meters below transducer surface.