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The objective of this project is to build a prototype of an Acoustic Microtome Imaging System (AMIS) that can be used to quantify changes in swim bladder shape and volume of swim bladder-bearing fish relative to pressure changes. The specific tasks of this project involves the following: 1. Design and fabricate the prototype of the system. 2. Conduct laboratory experiments to get 3D images of a number of fish at different depths. The expected outcomes from the proposed research include the following: 1. Obtain in situ and high resolution (~1mm) 3D acoustic images of fish swim bladders for both physoclistic and physostomous fish. 2. Present the results at a scientific meeting for the Working Group on Fisheries Acoustics Science and Technology (WGFAST). 3. Publish a peer-reviewed paper in a scientific journal (ICES JMS). Personnel involved in the projects are Drs. Dezhang Chu (PI), J. Michael Jech (NEFSC, co-PI), and Stan Tomich, Larry Hufnagle, and John Pohl from FRAM. Although this is a one-year project funded by the Advanced Sampling Technology Working Group (ASTWG) and was officially ended in 2010, the research will still be continued such as conducting measurements in laboratory and on board the vessel, and writing scientific papers. Raw binary data